St. Nicholas Church in Zhykhor neighbourhood was built between 1890 and 1900 according to a design by architect V. Nemkin, although the first St. Nicholas Church in the village was built in 1741.

The church also operated a parish school. In addition to the residents of Zhykhor, the parishioners included residents of the villages of Mokryi Zhykhor, Karachevka, and Guty.

The St. Nicholas (Svyato-Mykolayivsʹkyy) Church suffered severe damage during the Soviet era and World War II—the bell tower and dome were lost. The church building was later used as a movie theater and a grain storage facility.

Divine services resumed in the 1960s, and the church was restored in the 1990s and 2000s.

Today, Zhykhor is a district of Kharkiv. The church is located at Hetmanska Street, 11.